Focal Interest
Focal Interest

Real-time polymerase chain reaction

  1. Tongue swab PCR showed high accuracy for pulmonary tuberculosis
    Rapid PCR testing on tongue swabs shows promise for tuberculosis diagnosis in sputum-scarce patients, with high specificity but limitations in detecting low bacillary loads.
  2. Human Pol-PCNA binds DNA in an unexpected extra site
    Cryo-EM re-evaluation of human DNA polymerase epsilon-PCNA reveals unexpected multi-site DNA binding behavior contradicting prior mechanistic interpretations of mismatch recognition and proofreading.